To infect non-dividing cells, HIV must interact with and translocate the nuclear pore complex. The HIV-1 capsid protein (CA) is a viral determinant for nuclear entry and is known to interact directly with an FG repeat motif of the nucleoporin, Nup153. The nuclear pore complex is composed of multiple copies of around 30 different nucleoporins with at least 10 of these containing FG repeats. We are using a fluorescence-based assay to screen for interactions between recombinant HIV-1 CA spheres and novel FG repeat nucleoporins to better understand nuclear docking and entry.